Difference between revisions of "Part:BBa K339009"
 
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<partinfo>BBa_K339009 short</partinfo>
 
<partinfo>BBa_K339009 short</partinfo>
  
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The CpxR promoter coupled with a RFP promoter. This is attached to an arabinose inducible promoter, ribosome binding site, and mutant maltose binding protein.
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The CpxR promoter driving expression of red fluorescent protein (RFP). Downstream of this is an arabinose inducible promoter driving the expression of mutant maltose binding protein (malE31) with a storng ribosomal binding site.
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===Usage and Biology===
 
===Usage and Biology===
  
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malE31 is a mutant form of the maltose binding protein, a native protein to E''scherichia Coli''. 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K339009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K339009 SequenceAndFeatures</partinfo>

Latest revision as of 00:49, 31 October 2010

CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31

The CpxR promoter driving expression of red fluorescent protein (RFP). Downstream of this is an arabinose inducible promoter driving the expression of mutant maltose binding protein (malE31) with a storng ribosomal binding site.


Usage and Biology

malE31 is a mutant form of the maltose binding protein, a native protein to Escherichia Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2603
    Illegal BamHI site found at 2076
    Illegal BamHI site found at 2339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2301
    Illegal SapI site found at 1893