Difference between revisions of "Part:BBa K339006:Design"
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===Source=== | ===Source=== | ||
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− | + | The cpxP promoter was extracted from the <i>E. coli</i> genome through PCR using primers designed by literature (Shimohata et al. 2002). The XbaI restriction site was added in front of the gene and the full Biobrick suffix (SpeI, NotI, and PstI cut sites) were added to the end. | |
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===References=== | ===References=== | ||
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+ | Shimohata, N., Chiba, S., Saikawa, N., Ito, K., and Akiyama, A., 2002., The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. <i>Genes to cells</i> 7(7):653-662 | ||
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Revision as of 21:29, 30 October 2010
CpxP Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
The cpxP promoter was extracted from the E. coli genome through PCR using primers designed by literature (Shimohata et al. 2002). The XbaI restriction site was added in front of the gene and the full Biobrick suffix (SpeI, NotI, and PstI cut sites) were added to the end.
References
Shimohata, N., Chiba, S., Saikawa, N., Ito, K., and Akiyama, A., 2002., The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. Genes to cells 7(7):653-662