Difference between revisions of "Part:BBa K339010"
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<partinfo>BBa_K339010 short</partinfo> | <partinfo>BBa_K339010 short</partinfo> | ||
| − | Inclusion Body Binding Protein Promoter | + | Inclusion Body Binding Protein/ FxsA fusion Promoter |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This is a fusion promoter which fuses two heat shock promoters from E. Coli: Fxsa and ibpAB. Kraft et al (1997) designed this fusion promoter and found it to be considerably more sensitive to misfoled protein in the cytoplasm than either of the promoters alone. | |
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| + | The Fxsa promoter is not well documented. The ibpAB promoter controls the transcription of two small proteins: ibpA and ibpB. These are small heat shock proteins called inclusion body binding proteins. In the presence of inclusion bodies within the cytoplasm, they are thought to form mixed complexes, ibpA allowing ibpB to bind to the inclusoon body at higher temperatures. The binding of these proteins to the misfolded protein lowers its hydrophobicity, preventing further binding of exposed peptide chains, thus stabilizing the protein and mediating its refolding by the DnaK/DnaJ/GrpE chaperone protein system (Matuszewska at al., 2005). Transcription levels from this promoter have been found to increase the most upon heat shock as compared to other heat shock promoters (Chuang et al., 1993). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K339010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K339010 SequenceAndFeatures</partinfo> | ||
Revision as of 04:09, 30 October 2010
ibpAB
Inclusion Body Binding Protein/ FxsA fusion Promoter