Difference between revisions of "Part:BBa K339003"

Revision as of 02:06, 30 October 2010

malE31ΔSS

Mutant form of the Maltose binding protein (malE) with signal sequence deleted.

Usage and Biology

This mutant form of the maltose binding protein has the signal sequence deleted (amino acids). This results in the protein not benig localized to the periplasm of the cell, but rather staying in the cytoplasm of the cell. This mutant also has a BamHI site located at codon 32-33 replacing the wild-type sequence Gly-Ile. This two amino acid mutation destroys the folding capabilities of the protein, resulting in a non-folder.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 360
Illegal BamHI site found at 96
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 58

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K339003 short</partinfo>
-Mutant malE with the Signal Sequence Deleted
+Mutant form of the Maltose binding protein (malE) with signal sequence deleted.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This mutant form of the maltose binding protein has the signal sequence deleted (amino acids).  This results in the protein not benig localized to the periplasm of the cell, but rather staying in the cytoplasm of the cell.  This mutant also has a BamHI site located at codon 32-33 replacing the wild-type sequence Gly-Ile.  This two amino acid mutation destroys the folding capabilities of the protein, resulting in a non-folder.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K339003 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K339003 parameters</partinfo>
 <!-- -->