Difference between revisions of "Part:BBa K415508"
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<partinfo>BBa_K415508 short</partinfo> | <partinfo>BBa_K415508 short</partinfo> | ||
− | L4R1 Mammoblock Promoter Vector containing | + | L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter. |
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+ | ==Characterization== | ||
+ | [[Image:HEF1a_characterization.png|thumb|right|Figure 1. Fluorescence of HEK293FT cells 7 days post infection with hEF1a_EGFP construct.]] HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter. | ||
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Latest revision as of 18:00, 31 October 2010
phEF1a L4R1 MammoBlock Entry Vector
L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter.
Characterization
HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127 - 1000COMPATIBLE WITH RFC[1000]