Difference between revisions of "Part:BBa K415508"

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<partinfo>BBa_K415508 short</partinfo>
 
<partinfo>BBa_K415508 short</partinfo>
  
L4R1 Mammoblock Promoter Vector containing constitutive human elongation factor 1a (hEF1a) promoter.  
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L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter.
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==Characterization==
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[[Image:HEF1a_characterization.png|thumb|right|Figure 1. Fluorescence of HEK293FT cells 7 days post infection with hEF1a_EGFP construct.]] HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:00, 31 October 2010

phEF1a L4R1 MammoBlock Entry Vector

L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter.

Characterization

Figure 1. Fluorescence of HEK293FT cells 7 days post infection with hEF1a_EGFP construct.
HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter.

                                                                                                                                                                                                                                     


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]