Difference between revisions of "Part:BBa K305007"
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<partinfo>BBa_K305007 short</partinfo> | <partinfo>BBa_K305007 short</partinfo> | ||
| − | Promoter for the surfactin regulatory gene. Isolated from ''B.subtilis'' using a shortened region between the response element and the transcription start side. The promoter is activated by quorum sensing, a process which it influences itself, during the late exponential stage. It activates the formation of bifilm, sporulation and natural competence of ''B.subtilis''. | + | Promoter for the surfactin regulatory gene. Isolated from ''B.subtilis'' using a shortened region between the response element and the transcription start side. The promoter is activated by quorum sensing, a process which it influences itself, during the late exponential stage. It activates the formation of bifilm, sporulation and natural competence of ''B.subtilis''. Complete pathway to srfAA promoter linked to ComXPA signaling shown below. |
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| + | [Image:Image:Groningen-ComXPA-srfAA.png] | ||
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| + | [http://2010.igem.org/Team:Groningen#/expression iGEM Groningen 10] | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K305007 parameters</partinfo> | <partinfo>BBa_K305007 parameters</partinfo> | ||
| − | < | + | Promoter activity visualized by GFP expression using part <partinfo>BBa_E0240</partinfo> in liquid minimal medium. The growth curve is overlaid by the Fluorescence intensity to indicate the timing of the expression. Measurements performed in a Tecan microplate fluorometer. |
| + | [Image:SrfAA1prODvsGFP.png] | ||
Revision as of 20:52, 7 November 2010
srfA promoter, short variant
Promoter for the surfactin regulatory gene. Isolated from B.subtilis using a shortened region between the response element and the transcription start side. The promoter is activated by quorum sensing, a process which it influences itself, during the late exponential stage. It activates the formation of bifilm, sporulation and natural competence of B.subtilis. Complete pathway to srfAA promoter linked to ComXPA signaling shown below.
[Image:Image:Groningen-ComXPA-srfAA.png]
[http://2010.igem.org/Team:Groningen#/expression iGEM Groningen 10]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Promoter activity visualized by GFP expression using part BBa_E0240 in liquid minimal medium. The growth curve is overlaid by the Fluorescence intensity to indicate the timing of the expression. Measurements performed in a Tecan microplate fluorometer. [Image:SrfAA1prODvsGFP.png]