Difference between revisions of "Part:BBa K415504"

 
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<partinfo>BBa_K415504 short</partinfo>
 
<partinfo>BBa_K415504 short</partinfo>
  
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MIT iGEM 2010 Mammalian Part
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L1L2 Mammoblock entry vector containing the Rtta3_2a_hygro construct. rtTA3 is a DOX-inducible transcription factor controlling expression of the TRE promoter; Hygro is a selection marker against the Hygromycin antibiotic used to select for stable mammalian cell lines. They are separated by the viral 2A sequence which allows for polycistronic expression of genes on the same mRNA molecule.
  
 
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Revision as of 18:07, 28 October 2010

Rtta3_2a_Hygro L1L2 MammoBlock Entry Vector

L1L2 Mammoblock entry vector containing the Rtta3_2a_hygro construct. rtTA3 is a DOX-inducible transcription factor controlling expression of the TRE promoter; Hygro is a selection marker against the Hygromycin antibiotic used to select for stable mammalian cell lines. They are separated by the viral 2A sequence which allows for polycistronic expression of genes on the same mRNA molecule.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal PstI site found at 1462
    Illegal NotI site found at 2170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
    Illegal AgeI site found at 351
  • 1000
    COMPATIBLE WITH RFC[1000]