Difference between revisions of "Part:BBa K415512"

 
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<partinfo>BBa_K415512 short</partinfo>
 
<partinfo>BBa_K415512 short</partinfo>
  
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MIT iGEM 2010 Mammalian Part
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L4R1 MammoBlock Promoter Vector. Contains the cfos mechanosensitive promoter, which controls expression of the c-fos proto-oncogene in mammalian cells. Studies in the literature applying FSS of 800 µ demonstrated actin stress fiber formation and induction of mRNA for c-fos within an hour of application .
  
 
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Revision as of 19:07, 28 October 2010

pCFOS L4R1 MammoBlock Entry Vector

L4R1 MammoBlock Promoter Vector. Contains the cfos mechanosensitive promoter, which controls expression of the c-fos proto-oncogene in mammalian cells. Studies in the literature applying FSS of 800 µ demonstrated actin stress fiber formation and induction of mRNA for c-fos within an hour of application .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 168
    Illegal PstI site found at 541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 168
    Illegal PstI site found at 541
    Illegal NotI site found at 408
    Illegal NotI site found at 576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 168
    Illegal PstI site found at 541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 168
    Illegal PstI site found at 541
    Illegal NgoMIV site found at 572
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 314