Difference between revisions of "Part:BBa K313000"
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
 
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
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=== Verification Experiment ===
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Plasmid containing this part was transformed to Rosetta (DE3) plysS.
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When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM
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Fluorescence was measured and the results are shown below:
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:11, 2 November 2010

gfp generator with T7 promoter

gfp generator with T7 promoter

Usage and Biology

T7 RNA polymerase induces the expression of gfp from this part.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Verification Experiment

Plasmid containing this part was transformed to Rosetta (DE3) plysS. When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM Fluorescence was measured and the results are shown below:


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719