Difference between revisions of "Part:BBa K313008"
Line 7: Line 7:
  
 
We conducted recombination experiment using this part and Cre recombinase assay device.
 
We conducted recombination experiment using this part and Cre recombinase assay device.
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(Please see [Part:BBa_K313009 here].)
+
(Please see [https://parts.igem.org/Part:BBa_K313009 here].)
  
 
Now we are planning to conduct further research, seeking for appropriate terminator for 4C3 leak-switch.  
 
Now we are planning to conduct further research, seeking for appropriate terminator for 4C3 leak-switch.  

Revision as of 02:59, 28 October 2010

pT7 - Cre recombinase generator

It is the generator of the Cre recombinase.

Cre is transcribed by the T7 RNA polymerase.

We conducted recombination experiment using this part and Cre recombinase assay device. (Please see here.)

Now we are planning to conduct further research, seeking for appropriate terminator for 4C3 leak-switch.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 117
  • 1000
    COMPATIBLE WITH RFC[1000]