Difference between revisions of "Part:BBa K318516:Design"

Latest revision as of 02:02, 28 October 2010

RamA

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 116
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 116
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 116
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 116
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The ramA gene was PCR Amplified from Salmonella enterica strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts).

Source

The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.

References

Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.

Revision as of 01:16, 28 October 2010 (view source) Msagstetter (Talk \| contribs) (→‎Design Notes) ← Older edit		Latest revision as of 02:02, 28 October 2010 (view source) Msagstetter (Talk \| contribs) (→‎References)
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	===References===		===References===
		+
		+	Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in ''Salmonella enterica'' serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.