Difference between revisions of "Part:BBa K318516:Design"
Msagstetter (Talk | contribs) (→Design Notes) |
Msagstetter (Talk | contribs) (→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | The ramA gene was PCR Amplified from ''Salmonella enterica'' strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts) | + | The ramA gene was PCR Amplified from ''Salmonella enterica'' strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts). |
===Source=== | ===Source=== | ||
Revision as of 01:16, 28 October 2010
RamA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 116
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 116
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 116
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 116
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ramA gene was PCR Amplified from Salmonella enterica strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts).
Source
The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.