Difference between revisions of "Part:BBa K391007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on | + | We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1c3. |
| − | One out of two sets of primers included a histidine tag, which would be used to | + | One out of two sets of primers included a histidine tag, which would be used to purify DspB after it is expressed. |
| − | + | ||
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===Source=== | ===Source=== | ||
Latest revision as of 03:01, 28 October 2010
constitutive promoter-RBS-DspB (J23100:B0034:K391006)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1030
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1c3. One out of two sets of primers included a histidine tag, which would be used to purify DspB after it is expressed.
Source
The gene that encodes our protein, DspB, stems from the bacteria Actinobacillus actinomycetemcomitans.
References
1. Sigma Product Information for 4-Nitrophenyl N-acetyl-β-D-glucosaminide: [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/n9376pis.Par.0001.File.tmp/n9376pis.pdf Product Information]