Difference between revisions of "Part:BBa K322237:Experience"
(→Applications of BBa_K322237) |
(→Applications of BBa_K322237) |
||
| Line 5: | Line 5: | ||
===Applications of BBa_K322237=== | ===Applications of BBa_K322237=== | ||
[[Image:WTlucie-ph7.jpg]] | [[Image:WTlucie-ph7.jpg]] | ||
| + | |||
This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in. | This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in. | ||
[[Image:WTlucie-ph4.8.jpg]] | [[Image:WTlucie-ph4.8.jpg]] | ||
| + | |||
This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase. | This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase. | ||
Latest revision as of 00:56, 28 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K322237
This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in.
This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase.
User Reviews
UNIQ8e2da9504c6c4350-partinfo-00000000-QINU UNIQ8e2da9504c6c4350-partinfo-00000001-QINU