Difference between revisions of "Part:BBa K404319"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404319 short</partinfo>
 
<partinfo>BBa_K404319 short</partinfo>
 +
<br/><br><br>
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 +
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404319 [Cyan fluorescent protein]
 +
|-
 +
|'''BioBrick Nr.'''
 +
|[https://parts.igem.org/Part:BBa_K404319 BBa_K404319]
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
 +
|-
 +
|'''Requirement'''
 +
|pSB1C3<br>
 +
|-
 +
|'''Source'''
 +
|
 +
|-
 +
|'''Submitted by'''
 +
|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 +
|}
 +
<br><br><br><br><br><br><br><br><br>
 +
 +
  
 
<html>
 
<html>
Line 6: Line 28:
 
mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has a higher brightness level, acid insensivity and greater photostability.  
 
mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has a higher brightness level, acid insensivity and greater photostability.  
 
Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.   
 
Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.   
−
<html/>
+
<html/><br/>
 +
<html><head>
 +
  <meta http-equiv="Content-Type"
 +
content="text/html; charset=windows-1252">
 +
  <meta name="Generator"
 +
content="Microsoft Word 14 (filtered)">
 +
  <style>
 +
<!--
 +
/* Font Definitions */
 +
@font-face
 +
{font-family:Calibri;
 +
panose-1:2 15 5 2 2 2 4 3 2 4;}
 +
@font-face
 +
{font-family:Tahoma;
 +
panose-1:2 11 6 4 3 5 4 4 2 4;}
 +
/* Style Definitions */
 +
p.MsoNormal, li.MsoNormal, div.MsoNormal
 +
{margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:10.0pt;
 +
margin-left:0cm;
 +
line-height:115%;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";}
 +
a:link, span.MsoHyperlink
 +
{color:blue;
 +
text-decoration:underline;}
 +
a:visited, span.MsoHyperlinkFollowed
 +
{color:purple;
 +
text-decoration:underline;}
 +
p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
 +
{mso-style-link:"Balloon Text Char";
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
font-size:8.0pt;
 +
font-family:"Tahoma","sans-serif";}
 +
p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
 +
{margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:10.0pt;
 +
margin-left:36.0pt;
 +
line-height:115%;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";}
 +
p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
 +
{margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:0cm;
 +
margin-left:36.0pt;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";}
 +
p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
 +
{margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:0cm;
 +
margin-left:36.0pt;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";}
 +
p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
 +
{margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:10.0pt;
 +
margin-left:36.0pt;
 +
line-height:115%;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";}
 +
span.BalloonTextChar
 +
{mso-style-name:"Balloon Text Char";
 +
mso-style-link:"Balloon Text";
 +
font-family:"Tahoma","sans-serif";}
 +
.MsoChpDefault
 +
{font-family:"Calibri","sans-serif";}
 +
.MsoPapDefault
 +
{margin-bottom:10.0pt;
 +
line-height:115%;}
 +
@page WordSection1
 +
{size:612.0pt 792.0pt;
 +
margin:70.85pt 70.85pt 2.0cm 70.85pt;}
 +
div.WordSection1
 +
{page:WordSection1;}
 +
-->
 +
  </style>
 +
</head>
 +
<body lang="EN-US" link="blue" vlink="purple">
 +
<div class="WordSection1"><br>
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; width: 635px; height: 404px;"
 +
border="1" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 252.85pt;">
 +
      <td
 +
style="border: 1pt solid windowtext; padding: 0cm 5.4pt; width: 481.1pt; height: 252.85pt;"
 +
valign="top" width="641">
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
      <img style="width: 324px; height: 262px;" alt=""
 +
id="Picture 2"
 +
src="https://static.igem.org/mediawiki/2010/f/f5/Freiburg10_CFP_spectrum.png"></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">Fig.1:
 +
Emission spectra and absorbance CFP</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
style="font-size: 9pt;" lang="DE"><a
 +
href="http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg">http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg</a></span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      </td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<p class="MsoNormal">&nbsp;</p>
 +
<p class="MsoNormal">&nbsp;</p>
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; width: 416px; height: 365px;"
 +
border="1" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 261.8pt;">
 +
      <td
 +
style="border: 1pt solid windowtext; padding: 0cm 5.4pt; width: 470.8pt; height: 261.8pt;"
 +
valign="top" width="628">
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">
 +
      <table style="width: 358px; height: 256px;" align="left"
 +
cellpadding="0" cellspacing="0">
 +
        <tbody>
 +
          <tr>
 +
            <td>&nbsp; &nbsp; &nbsp; &nbsp;
 +
&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;
 +
&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<img
 +
style="width: 349px; height: 233px;" alt=""
 +
src="https://static.igem.org/mediawiki/2010/9/98/Freiburg10_CFP_fluorophor.png"></td>
 +
          </tr>
 +
        </tbody>
 +
      </table>
 +
      <span lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;&nbsp;&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE">&nbsp;</span></p>
 +
      <br clear="all">
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">Fig.2:Chromophor
 +
structure of CFP</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE"><a
 +
href="http://jcs.biologists.org/cgi/reprint/120/24/4247"><span
 +
lang="EN-US">http://jcs.biologists.org/cgi/reprint/120/24/4247</span></a></span></p>
 +
      </td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<p class="MsoNormal">&nbsp;</p>
 +
<p class="MsoNormal">&nbsp;</p>
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; width: 643px; height: 459px;"
 +
border="1" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 320.75pt;">
 +
      <td
 +
style="border: 1pt solid windowtext; padding: 0cm 5.4pt; width: 465.2pt; height: 320.75pt;"
 +
valign="top" width="620">
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
style="position: relative; z-index: 251671552;"><span
 +
style="position: absolute; left: 149px; top: -2px; width: 304px; height: 350px;"><img
 +
style="width: 304px; height: 350px;" alt=""
 +
src="https://static.igem.org/mediawiki/2010/b/b8/Freiburg10_CFP_architecture.png"></span></span>&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">&nbsp;</p>
 +
      <br clear="all">
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;">Fig.3:&nbsp;
 +
Fluorescent protein beta-barrel architecture and approximate dimensions</p>
 +
      <p class="MsoNormal"
 +
style="margin-bottom: 0.0001pt; line-height: normal;"><span
 +
lang="DE"><a
 +
href="http://jcs.biologists.org/cgi/reprint/120/24/4247">http://jcs.biologists.org/cgi/reprint/120/24/4247</a></span></p>
 +
      </td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<p class="MsoNormal"><span lang="DE">&nbsp;</span></p>
 +
<p class="MsoNormal"><span lang="DE">&nbsp;</span></p>
 +
</div>
 +
</body>
 +
</html>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:55, 27 October 2010

Cyan fluorescent protein (CFP) in RFC25 standard


[Cyan fluorescent protein
BioBrick Nr. BBa_K404319
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]











Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has a higher brightness level, acid insensivity and greater photostability. Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.


 

 

 

                                        

 

Fig.1: Emission spectra and absorbance CFP

 

http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg

 

 

 

 

 

                             
 

   

  

 

 

 

 

 

 

 

 

 

 

 

 


Fig.2:Chromophor structure of CFP

http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Fig.3:  Fluorescent protein beta-barrel architecture and approximate dimensions

http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]