Difference between revisions of "Part:BBa K395701"
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It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤ | It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤ | ||
| − | [[Image: | + | [[Image:Tokyotech_registry-zeaxanthin.jpg|right|500px|thumb|Fig.2 zeaxanthin pellet and plate, TLC<br>These works are done by Yumiko Kinoshita]] |
Revision as of 23:16, 27 October 2010
rbs+crtZ under plac promoter for double plasmids
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤
File:Tokyotech registry-zeaxanthin.jpg
Fig.2 zeaxanthin pellet and plate, TLC
These works are done by Yumiko Kinoshita
These works are done by Yumiko Kinoshita
These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]