Difference between revisions of "Part:BBa K374006:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | =Characterization of BBa_K374006= | ||
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| + | == Positive feedback antitermination on double reporter plasmid == | ||
| + | The iGEM DTU team 2010 have submitted this part, after using it in the development of a genetic bistable switch. | ||
| + | |||
| + | === Aim and strategy === | ||
| + | The aim of the characterization was to asses if this part together with <partinfo>BBa_K374005 </partinfo> could have an antiterminating function that could be used as a regulatory mechanism. | ||
| + | |||
| + | We characterized the functionality of the part in the following construct. | ||
| + | [[Image:example.jpg|center|frame|caption]] | ||
| + | |||
| + | XXXXXXXXXXXXXXXXplenation to the figure and the parts it consists of. | ||
| + | |||
| + | we tested if: | ||
| + | * If it was possible to trigger a positive feedback mechanism of N protein, by varying the promoter strength with the synthetic promoter library (SPL) and thus antitermination and constant expression of the down stream RFP. | ||
| + | * If it was possible to trigger this positive feed back mechanism by inducing the N protein from a second plasmid insert with the pBAD promoter and the N protein. | ||
| + | |||
| + | === Results === | ||
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| + | |||
| + | |||
| + | ==== Conclusionc and Recommendations ==== | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 22:03, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterization of BBa_K374006
Positive feedback antitermination on double reporter plasmid
The iGEM DTU team 2010 have submitted this part, after using it in the development of a genetic bistable switch.
Aim and strategy
The aim of the characterization was to asses if this part together with BBa_K374005 could have an antiterminating function that could be used as a regulatory mechanism.
We characterized the functionality of the part in the following construct.
XXXXXXXXXXXXXXXXplenation to the figure and the parts it consists of.
we tested if:
- If it was possible to trigger a positive feedback mechanism of N protein, by varying the promoter strength with the synthetic promoter library (SPL) and thus antitermination and constant expression of the down stream RFP.
- If it was possible to trigger this positive feed back mechanism by inducing the N protein from a second plasmid insert with the pBAD promoter and the N protein.
Results
Conclusionc and Recommendations
User Reviews
UNIQ49759eab1b8cb071-partinfo-00000001-QINU
|
BBa_K374006 1 Not understood iGEM DTU 2010 |
We have characterized this part. |
UNIQ49759eab1b8cb071-partinfo-00000003-QINU