Difference between revisions of "Part:BBa K361001"
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<partinfo>BBa_K361001 short</partinfo>
 
<partinfo>BBa_K361001 short</partinfo>
  
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This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.
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This message system consists of a message sequence, with specific repeat sequences inserted as to be recognized by the Rci site-specific recombinase.<br>
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*This message has 70 characters - "We must learn to live together as brothers or perish together as tools"<br>
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*In a 4 bits system, the total sequence is 280 bp.<br>
  
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*This message has 70 characters:
 
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We must learn to live together as brothers or perish together as tools
 
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*In a 4 bits system, the total sequence is 280 bp.
 
 
In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
 
In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
  
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*Reference:
 
*Reference:
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Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.
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[1] Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:42, 27 October 2010

Message gene template

This message system consists of a message sequence, with specific repeat sequences inserted as to be recognized by the Rci site-specific recombinase.

  • This message has 70 characters - "We must learn to live together as brothers or perish together as tools"
  • In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

Msg.png


  • Reference:

[1] Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
    Illegal BamHI site found at 427
    Illegal XhoI site found at 433
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]