Difference between revisions of "Part:BBa K374006"

Revision as of 00:48, 28 October 2010

Lambda N anti-terminator.

This part contains the coding sequence for the lambda N anti terminator protein, which will suppress intrinsic transcription termination downstream of the nut site a regulatory sequence, see BBa_K374005.

K374005

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native E. coli regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].

This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application in construction of a bistable switch.

references

[1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
[2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009.
[3] Whalen. W., Ghosh. B., Das. A., "NusA protein is necessary and sufficient in vitro for phage lambda N gene product to suppress a rho-independent terminator placed downstream of nutL. Proc.Natl. Acad sci. 1988

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This part contains the coding sequence for the lambda N anti terminator protein, which will suppress intrinsic transcription termination downstream of the nut site a regulatory sequence, see BBa_K374005.<partinfo>BBa_K374005 SpecifiedComponents</partinfo>
-In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native ''e. coli'' regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1]
+In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native ''E. coli'' regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].
-Antitermination have been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].
+This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application in construction of a bistable switch.
-This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark DTU igem team 2010], see our wiki  for further description of the parts and for an example of application in construction of a bistable switch.
 === references ===