Difference between revisions of "Part:BBa K318513"
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===Usage and Biology===
 
===Usage and Biology===
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===Low pH/Stationary Phase Induction===
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Download testing procedure (.pdf) [[Media:GadAp_Testing_Procedure.pdf|here]].
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Download example spreadsheet (.xls) [[Media:Copy of Final Friday Eval.xls|here]].
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Below are two figures of sample data:
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[[Image:pH_7_gadAp_with_controls.jpg|center]]
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'''Figure 4.''' Lines represent OD600 and are associated with the right axis. Dots indicate RFU/OD600 and associated with the left axis. In this trial, maximum induction occurred at an OD600 of approximately 6 (right axis, associated with line) and yielded about 10000 RFU/OD. Note that immediately before maximum OD600 was reached, RFU/OD increased about 5-fold. Controls included for this trial were a lacI promoter + RBS + mRFP1 + TT construct in the same plasmid (pSB1A2) and a WT control of MG1655 cells. These controls and results indicate that (1) mRFP1 expression by gadAp at high OD600 is a function of the gadA promoter, not of promoters at large (lacI promoter did not express) or of the cells themselves (MG1655 did not fluoresce).
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[[Image:pH_5.5_gadAp_with_controls.jpg|center]]
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'''Figure 5.'''This trial was run parallel to the pH 7 trial in Figure 4. It indicates that cells grown in LB media at pH 5.5 do not exhibit a drastically different promotion behavior from their gadA promoters than those grown in neutral media.
  
 
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Revision as of 02:01, 28 October 2010

gadA/RBS + RFP + TT

uc

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 825
    Illegal AgeI site found at 937
  • 1000
    COMPATIBLE WITH RFC[1000]