Difference between revisions of "Part:BBa K404008"

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	<h3>References</h3>		<h3>References</h3>
−	<b>Cassinotti, P., Weitzand, M., & Tratschin, J. D.</b>, 1988. Organization of the adeno-associated virus (AAV) capsid gene: mapping of a minor spliced mRNA coding for virus capsid protein. Virology, 167(1), 176-84 <br />	+	<b>Bleker, Pawlita, & Kleinschmidt</b>, 2006. Impact of capsid conformation and rep-capsid interactions on adeno-associated virus type 2 genome packaging. Journal of Virology, 810–820 <br />
	<center><img src="https://static.igem.org/mediawiki/parts/a/a7/Freiburg10_Cap_proteins_VP1_2%263.png" width="600"		<center><img src="https://static.igem.org/mediawiki/parts/a/a7/Freiburg10_Cap_proteins_VP1_2%263.png" width="600"

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Revision as of 20:49, 27 October 2010

[AAV2]-VP1

AAV2-VP1
BioBrick Nr.	BBa_K404008
RFC standard	RFC 25
Requirement	pSB1C3_001
Source	pAAV_RC from Stratagene
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

The viral capsid is formed by the three structural proteins VP1, VP2 and VP3 which are encoded by the cap gene in an overlapping reading frame. They form an icosahedral symmetry arranged in a stoichiometric ratio of 1:1:10. The mRNA encoding for the cap proteins is transcribed from p40 and alternative spliced to minor and major products. Sharing a common C terminus and stop codon, the VP proteins begin with a different start codon. The translation of VP2 from the major spliced mRNA is not as efficient as of VP1 and VP3 because it initiates at a Thr codon (ACG). The N terminus of VP2 contains a motiv of about 70 amino acids that is highly homologous to a phospholipase A2 (PLA2) domain. Furthermore, there are nuclear localization sequences (BR)(+) which are supposed to be necessary for endosomal escape and nuclear entry. The exact role of VP2 remains unknown, although the protein is thought to be nonessential for viral assembly and infectivity.

References

Bleker, Pawlita, & Kleinschmidt, 2006. Impact of capsid conformation and rep-capsid interactions on adeno-associated virus type 2 genome packaging. Journal of Virology, 810–820
Figure 1: The VP proteins are encoded in an overlapping open reading frame. .
Sequence and Features