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Revision as of 18:07, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I716211
10/27/10: This part induces cell death when expressed. We (the 2010 Harvard iGEM team) received this part from the Anderson lab at Berkeley as part of an arabinose-inducible Barnase device that also contained a constitutive Barstar. Barstar is another protein that prevents Barnase from killing the cell. In our experience Barstar is required to even be able to clone Barnase, because Barnase is so potent.
User Reviews
UNIQ7abe38f9407487a1-partinfo-00000000-QINU
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BBa_I716211 1 Not understood Pmboyle |
10/27/10: Part works well, arabinose inducible Barnase caused cell death in a dose dependent manner. Please see http://2010.igem.org/Team:Harvard/fences/results for more info. |
UNIQ7abe38f9407487a1-partinfo-00000002-QINU