Difference between revisions of "Part:BBa K398029"
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[http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results TU Delft 2010 results] suggest that the cell extracts obtained from the recombinant strain ''E. coli'' 029A, which expresses this part, have a dodecanal dehydrogenase activity twice as high as the control strain (''E. coli'' with the empty plasmid pSB1A2). This activity is equivalent to 33.98% of the cell extract of ''Pseudomonas putida'' growing on octane as sole carbon source. | [http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results TU Delft 2010 results] suggest that the cell extracts obtained from the recombinant strain ''E. coli'' 029A, which expresses this part, have a dodecanal dehydrogenase activity twice as high as the control strain (''E. coli'' with the empty plasmid pSB1A2). This activity is equivalent to 33.98% of the cell extract of ''Pseudomonas putida'' growing on octane as sole carbon source. | ||
| − | [[Image:TUDelftALDH_final.jpg| | + | [[Image:TUDelftALDH_final.jpg|470px|thumb|left|Comparison of ALDH activities in the different strains tested in this study]] |
Revision as of 16:57, 27 October 2010
ALDH generator (low expression)
ALDH is an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids (see figure 1), which can then be further degraded through β-oxidation
The [http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation characterization of BBa_K398029] has been described on the TU Delft iGEM Team 2010 wiki.
Usage and Biology
Aldehyde dehydrogenase from the thermophile Geobacillus thermoleovorans B23. It functions as an octamer, requiring NAD+ as coenzyme. The optimum condition for activity lies at temperatures between 50 and 55 degrees celsius and and a pH of 10. You can see our original plasmid map below.
Characterization
This part was characterized at 37º C and pH=9.5, using recombinant strains carrying this part on the plasmid pSB1A2 by TU Delft 2010 iGEM team.
Results
[http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results TU Delft 2010 results] suggest that the cell extracts obtained from the recombinant strain E. coli 029A, which expresses this part, have a dodecanal dehydrogenase activity twice as high as the control strain (E. coli with the empty plasmid pSB1A2). This activity is equivalent to 33.98% of the cell extract of Pseudomonas putida growing on octane as sole carbon source.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
- 1000COMPATIBLE WITH RFC[1000]
[1] Kato, T., et al., Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23. Extremophiles, 2010. 14(1): p. 33-39.