Difference between revisions of "Part:BBa K395402:Experience"
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===Applications of BBa_K395402=== | ===Applications of BBa_K395402=== | ||
In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part. <br> | In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part. <br> | ||
| − | We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1. | + | We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br> |
| − | + | ||
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2. | We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2. | ||
Revision as of 13:05, 27 October 2010
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Applications of BBa_K395402
In order to measure LacIM1(BBa_K082026) and LacI(BBa_K395400), we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.
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