Difference between revisions of "Part:BBa K323132:Experience"
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[[Image:Sloviolaceinextracts.JPG|right|thumb|420px| '''Figure: Violacein extracts of bacterial cultures carrying parts BBa_K323132 and BBa_K323135 and containing a DNA program or a scrambled DNA program or no DNA program.''' Violacein was extracted from the incubated cultures. An extensive difference in color intensity is visible among the extracts of all three cultures, among which the extract of the culture containing a plasmid with the DNA program exhibits the most intensive purple color.]] | [[Image:Sloviolaceinextracts.JPG|right|thumb|420px| '''Figure: Violacein extracts of bacterial cultures carrying parts BBa_K323132 and BBa_K323135 and containing a DNA program or a scrambled DNA program or no DNA program.''' Violacein was extracted from the incubated cultures. An extensive difference in color intensity is visible among the extracts of all three cultures, among which the extract of the culture containing a plasmid with the DNA program exhibits the most intensive purple color.]] | ||
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Revision as of 13:05, 27 October 2010
Cells containing parts with Part:BBa_K323135 and Part:BBa_K323132 were transformed with Part:BBa_K323066 or Part:BBa_K323153 or a commercial pBluescript plasmid without a DNA program. Cultures were incubated at 30°C for several days and samples were taken at various times. Violacein was extraced from individual samples to analyze the products and evaluate the yield of violacein production.
By measuring the concentrations of violacein in the extracts of taken samples, we determined that yield of violacein production in our system increased 6-fold in comparison to an E. coli strain containing a plasmid without a DNA program.
In addition, the 2010 iGEM team Slovenia have shown that a DNA program-mediated biosynthesis decreases formation of an unwanted side product deoxychromoviridans. We have analysed the products of the vio operon (Part:Bba_K274002) with HPLC, TLC and mass spectrometry (for detailed description see the 2010 iGEM team Slovenia [http://2010.igem.org/Team:Slovenia/METHODS_and_PARTS wiki]).
Applications of BBa_K323132
User Reviews
UNIQ770d0ff1d0ceeb25-partinfo-00000000-QINU UNIQ770d0ff1d0ceeb25-partinfo-00000001-QINU