Difference between revisions of "Part:BBa C0056"
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<partinfo>BBa_C0056 parameters</partinfo>
 
<partinfo>BBa_C0056 parameters</partinfo>
 
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<h2>Characteration </h2>
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<p> In 2019, UCAS-China made several improvements to CI434, BBa_C0056 by constructing a new part, CI434-TEVsite, . Firstly, we inserted the TEV protease recognition sequence between N-terminal and C-terminal of CI434 so that it can be cleaved by TEV and then lose activity, which can regulate gene expression at both transcriptional and proteolytic levels. As shown in Figure 1 and Figure 2, we can see that CI434-TEVsite and original TEV have little difference. However, when there exists TEV protease, CI434-TEVsite can lose its activity so that the genes expression under CI434-TEVsite can be activated significantly.
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</p>

Revision as of 13:05, 19 October 2019


cI repressor from phage 434 (no LVA)

-- No description --

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characteration

In 2019, UCAS-China made several improvements to CI434, BBa_C0056 by constructing a new part, CI434-TEVsite, . Firstly, we inserted the TEV protease recognition sequence between N-terminal and C-terminal of CI434 so that it can be cleaved by TEV and then lose activity, which can regulate gene expression at both transcriptional and proteolytic levels. As shown in Figure 1 and Figure 2, we can see that CI434-TEVsite and original TEV have little difference. However, when there exists TEV protease, CI434-TEVsite can lose its activity so that the genes expression under CI434-TEVsite can be activated significantly.