Difference between revisions of "Part:BBa K320001:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The DNA was optimized for synthesis in ''E. coli'' and Asaia.
+
The DNA was optimized for synthesis in ''E. coli'' using the Mr. Gene programm. The sequence should also work better in ''Asaia''.
  
 
===Source===
 
===Source===

Revision as of 15:51, 27 October 2010

Immunotoxin against P.Berghei


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 957
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472
    Illegal BsaI site found at 699


Design Notes

The DNA was optimized for synthesis in E. coli using the Mr. Gene programm. The sequence should also work better in Asaia.

Source

Coding sequence comes from [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X [1]].

References

  1. [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]