Difference between revisions of "Part:BBa K320005:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K320005=== | ===Applications of BBa_K320005=== | ||
| − | We used this BioBrick to characterize | + | We used this BioBrick to characterize a strong promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br /> |
Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | ||
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a. | The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a. | ||
Revision as of 14:35, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
Applications of BBa_K320005
We used this BioBrick to characterize a strong promoter we want to use in Asaia BBa_K320002. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.
Both measurements were done simultaneously in the E. coli strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.
User Reviews
UNIQ04ec8b3c434b199c-partinfo-00000001-QINU UNIQ04ec8b3c434b199c-partinfo-00000002-QINU