Difference between revisions of "Part:BBa K320005:Experience"
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We used this BioBrick to characterize the promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br /> | We used this BioBrick to characterize the promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br /> | ||
Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br /> | ||
| − | The | + | The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a. |
Revision as of 11:47, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K320005
We used this BioBrick to characterize the promoter we want to use in Asaia BBa_K320002. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.
Both measurements were done simultaneously in the E. coli strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.
User Reviews
UNIQ7272c91130d270ee-partinfo-00000001-QINU UNIQ7272c91130d270ee-partinfo-00000002-QINU