Difference between revisions of "Part:BBa K317024"
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In this device, Antigen 43 is expressed constitutively under a strong promoter. The result of measuring OD660 approximately 1 cm from the top of the culture solution is shown below (Fig). | In this device, Antigen 43 is expressed constitutively under a strong promoter. The result of measuring OD660 approximately 1 cm from the top of the culture solution is shown below (Fig). | ||
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It shows the ''E. coli'', which harbors the plasmid encodes this device, the OD660 decreased. Meanwhile, the control didn’t show remarkable decrease. This means ''E. coli'' aggregates from the expression of Antigen 43. | It shows the ''E. coli'', which harbors the plasmid encodes this device, the OD660 decreased. Meanwhile, the control didn’t show remarkable decrease. This means ''E. coli'' aggregates from the expression of Antigen 43. | ||
Revision as of 11:48, 27 October 2010
Constitutive promoter(BBa_J23102)-RBS-Antigen 43-Double terminator
In this device, Antigen 43 is expressed constitutively under a strong promoter. The result of measuring OD660 approximately 1 cm from the top of the culture solution is shown below (Fig).
It shows the E. coli, which harbors the plasmid encodes this device, the OD660 decreased. Meanwhile, the control didn’t show remarkable decrease. This means E. coli aggregates from the expression of Antigen 43.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 1994
Illegal NgoMIV site found at 2504
Illegal NgoMIV site found at 2525
Illegal AgeI site found at 1520
Illegal AgeI site found at 2264
Illegal AgeI site found at 2678
Illegal AgeI site found at 2920 - 1000COMPATIBLE WITH RFC[1000]