Difference between revisions of "Part:BBa K336053"
Line 3: | Line 3: | ||
To obtain large amount of protein, over expression system was designed. | To obtain large amount of protein, over expression system was designed. | ||
− | Expression of T7 polymerase< | + | Expression of T7 polymerase<https://parts.igem.org/Part:BBa_K145001>is induced by IPTG. |
Add IPTG when you need, you obtain target of protein at appropriate phase. | Add IPTG when you need, you obtain target of protein at appropriate phase. | ||
Revision as of 11:46, 27 October 2010
Over expression system (T7 polymerase induced by lac promoter)
To obtain large amount of protein, over expression system was designed. Expression of T7 polymerase<https://parts.igem.org/Part:BBa_K145001>is induced by IPTG. Add IPTG when you need, you obtain target of protein at appropriate phase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]