Difference between revisions of "Part:BBa K320004"
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 This BioBrick consists of an origin of replication for Asaia and E. coli  (<partinfo>BBa_K320000</partinfo>)  and a resistance to Kanamycin (<partinfo>BBa_P1003</partinfo>) . It was initially designed to be inserted into (<partinfo>BBa_I51020 </partinfo>).  
 

 This BioBrick consists of an origin of replication for Asaia and E. coli  (<partinfo>BBa_K320000</partinfo>)  and a resistance to Kanamycin (<partinfo>BBa_P1003</partinfo>) . It was initially designed to be inserted into (<partinfo>BBa_I51020 </partinfo>).  
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We devellopped this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia'']
  
 
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Revision as of 16:00, 27 October 2010

Origin for Asaia and resistance to Kanamycin



 This BioBrick consists of an origin of replication for Asaia and E. coli (BBa_K320000) and a resistance to Kanamycin (BBa_P1003) . It was initially designed to be inserted into (BBa_I51020).

We devellopped this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4