Difference between revisions of "Part:BBa K328003"
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Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J. | Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J. | ||
Biol. Chem. 272, 196–202. | Biol. Chem. 272, 196–202. | ||
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| + | This part was improved upon as https://parts.igem.org/Part:BBa_K2282011 where it was used for conditional expression of an amilCP reporter only at low temperature (~15 C). | ||
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Latest revision as of 08:51, 31 October 2017
cspA Cold box + UTR + SD +DB
This is Cold Box + UTR + SD + DB to test it with other promoters and measure them. More info in BBa_K328001
Warning!. The use of an Dowstream Box sequence in a coding device could lead the cell to a state of a "low temperature antibiotic effect (LACE)" by truncated RNA with the DB sequence. For more info see:
Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J. Biol. Chem. 272, 196–202.
This part was improved upon as https://parts.igem.org/Part:BBa_K2282011 where it was used for conditional expression of an amilCP reporter only at low temperature (~15 C).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]