Difference between revisions of "Part:BBa K398406"

Revision as of 15:22, 27 October 2010

Solvent tolerance cluster BBa_K398406 enables E.coli of working in two-phase systems with resistance to high concentrations of hydrophobic molecules in the membrane. A growth rate increase of 62% in comparison to a standard E.coli was achieved at concentrations of 8% (v/v) n-hexane.

File:Protein 2ZDI.gif

Figure 1 - Proteins [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2ZDI phPFDα and phPFDβ]

Introduction

The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from Pyrococcus horikoshii OT3. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes.

File:TU Delft SaltModel.jpg

figure 1: Model of the Solvent tolerance

The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 37
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K398406 short</partinfo>
+BBa_K398406 enables ''E.coli'' of working in two-phase  systems with resistance to high concentrations of hydrophobic molecules in the membrane. A growth rate increase of 62% in comparison to a standard ''E.coli'' was achieved at concentrations of 8% (v/v) n-hexane.
-Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12.
+[[Image:Protein_2ZDI.gif|right|thumb|250px|'''Figure 1''' - Proteins [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2ZDI phPFDα and phPFDβ]]]
+==Introduction==
+The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from ''Pyrococcus horikoshii OT3''. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes.
+[[Image:TU Delft SaltModel.jpg|500px|center|thumb|figure 1: Model of the Solvent tolerance]]
 The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.