Difference between revisions of "Part:BBa K364325:Experience"

Revision as of 07:56, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Ligand

PXR is activated by a large number of endogenous and exogenous chemicals including steroids, antibiotics, antimycotics, bile acids, hyperforin, a constituent of the herbal antidepressant St. John's Wort, and many other herbal compounds.

PXR is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.

Physiological function of PXR

Like other type II nuclear receptors, when activated, it forms a heterodimer with the retinoid X receptor, and binds to hormone response elements on DNA which elicits expression of gene products.

One of the primary targets of PXR activation is the induction of CYP3A4, an important phase I oxidative enzyme that is responsible for the metabolism of many drugs. In addition, PXR up regulates the expression of phase II conjugating enzymes such as glutathione S-transferase and phase III transport uptake and efflux proteins such as OATP2 (SLCO2A1)and MDR1(ABCB1).

Possible applications of GAL4-PXR

By the addition of lipid ligand, the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364325

Picture of gel electrophoresis: Gal4-PXR in pCDNA3.1 resulting an insert of 1181 bp.

Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1

Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1

Test of pCDNA/pSB1A3-GAL4-PXR/EcR-LBD activity

Test of PXR:RXR interaction

User Reviews

UNIQ7db293048c19e27d-partinfo-00000000-QINU UNIQ7db293048c19e27d-partinfo-00000001-QINU