Difference between revisions of "Part:BBa C0062"

Revision as of 14:43, 14 July 2008

luxR repressor/activator, (no LVA?)

In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Usage and Biology

Unspecified.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_C0062 short</partinfo>
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 In complex with HSL, LuxR binds to the Lux promoter, activating transcription      from Pr <bbpart>BBa_R0062</bbpart>, and repressing transcription from Pl <bbpart>BBa_R0063</bbpart>.
-The lux cassette of V. fischeri contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the Lux activator, LuxR complexed      to HSL. Two molecules of LuxR protein form a complex with two molecules the      signalling compound homoserine lactone (HSL). This complex binds to a palindromic      site on the promoter, increasing the rate of transcription.
+The lux cassette of ''V. fischeri'' contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the Lux activator, LuxR complexed      to HSL. Two molecules of LuxR protein form a complex with two molecules the      signalling compound homoserine lactone (HSL). This complex binds to a palindromic      site on the promoter, increasing the rate of transcription.