Difference between revisions of "Part:BBa K395705"
Line 5: Line 5:
  
 
Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.  
 
Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.  
 +
 +
[[Image:tokyotech_registry-metabolite706.jpg|left|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
  
 
[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
 
[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
−
[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|500px|Fig. 2-1-12-2 astaxanthin extract]]
+
[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|460px|Fig. 2-1-12-2 astaxanthin extract]]
  
 
[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
 
[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
 
[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|400px|Fig. 2-1-1-3.TLC.jpg]]
 
[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|400px|Fig. 2-1-1-3.TLC.jpg]]
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
  
  

Revision as of 05:56, 27 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.

Fig. 2-1-11-2 astaxanthin pellet and plate
Fig. 2-1-11-2 astaxanthin pellet and plate
Fig. 2-1-12-2 astaxanthin extract
figure2
Fig. 2-1-1-3.TLC.jpg
















These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
































Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715
    Illegal SapI site found at 542
    Illegal SapI site found at 554