Difference between revisions of "Part:BBa K395400:Experience"
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===Applications of BBa_K395400=== | ===Applications of BBa_K395400=== | ||
| − | LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. Though this part was registered by USTC(2008), it was not well characterized in their wiki. Therefore, we characterized this part. | + | LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. Though this part was registered by USTC(2008), it was not well characterized in their wiki. Therefore, we characterized this part.<br><br> |
| + | We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])<br> | ||
| + | We ligated BBa_K395400 together with Pbad/araC promoter. | ||
| + | Then, we used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). | ||
| + | Fluorescence was measured, and the result is shown in Fig. 3-2-2. | ||
| + | [[Image:Tokyotech_LacIM1_system_ver4.png|left|thumb|300px|Figure3-2-1. ]] | ||
| + | [[Image:Tokyotech_LacIM1_data_ver2.png|center|thumb|300px|Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera ]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:50, 27 October 2010
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K395400
LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. Though this part was registered by USTC(2008), it was not well characterized in their wiki. Therefore, we characterized this part.
We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We ligated BBa_K395400 together with Pbad/araC promoter.
Then, we used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1).
Fluorescence was measured, and the result is shown in Fig. 3-2-2.
User Reviews
UNIQ01a5e47c6c0fd535-partinfo-00000000-QINU UNIQ01a5e47c6c0fd535-partinfo-00000001-QINU