Difference between revisions of "Part:BBa K337031"
| Line 2: | Line 2: | ||
<partinfo>BBa_K337031 short</partinfo> | <partinfo>BBa_K337031 short</partinfo> | ||
| − | RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by a double Tet operator. This part can be used for regulation the expressio of any GOI via the Tet System. | + | RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by a double Tet operator. This part can be used for regulation the expressio of any GOI via the Tet System (BB-2, RFC12). |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:17, 27 October 2010
BGH(rc)/shRNA6(rc)/RSV(rc)/CMV_TetO2(fw)
RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by a double Tet operator. This part can be used for regulation the expressio of any GOI via the Tet System (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1659
Illegal BamHI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]