Difference between revisions of "Part:BBa K349007:Experience"

(→‎Applications of BBa_K349007)
 
Line 4: Line 4:
  
 
===Applications of BBa_K349007===
 
===Applications of BBa_K349007===
−
Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are necessary for use in the BioBytes 2.0 assembly method.
+
Digestion with BsaI around the recognition sites inside of the prefix and suffix will create a kan cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the kan cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 14:20, 6 November 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K349007

Digestion with BsaI around the recognition sites inside of the prefix and suffix will create a kan cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the kan cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.

User Reviews

UNIQ8e76adc01c1bb25c-partinfo-00000000-QINU UNIQ8e76adc01c1bb25c-partinfo-00000001-QINU