Difference between revisions of "Part:BBa K349006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part has built-in | + | This part has built-in BfuAI cutsites surrounding the J00450 RFP cassette. Cutting at the BfuAI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010. |
===Source=== | ===Source=== |
Latest revision as of 02:48, 27 October 2010
RFP-BA-BfuAI (for construction of BA BioBytes 2.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has built-in BfuAI cutsites surrounding the J00450 RFP cassette. Cutting at the BfuAI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010.
Source
pSB1C3