Difference between revisions of "Part:BBa K337021"
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<partinfo>BBa_K337021 short</partinfo> | <partinfo>BBa_K337021 short</partinfo> | ||
| − | SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the | + | SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the CMV promoter. (BB-2, RFC12) |
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Latest revision as of 03:31, 27 October 2010
BGH(rc)/shRNA10(rc)/sv40(rc)/CMV(fw)
SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the CMV promoter. (BB-2, RFC12)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]