Difference between revisions of "Part:BBa K349302:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part has built-in BsaI | + | This part has built-in BsaI recognition sites surrounding a P1002 ampicillin resistance cassette. Digesting at the BsaI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioBytes 2.0 assembly method. |
===Source=== | ===Source=== |
Latest revision as of 04:03, 27 October 2010
ampR-BA (BioBytes 2.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 960
Design Notes
This part has built-in BsaI recognition sites surrounding a P1002 ampicillin resistance cassette. Digesting at the BsaI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioBytes 2.0 assembly method.
Source
pSB1C3