Difference between revisions of "Part:BBa K399001:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K399001 short</partinfo>
 
<partinfo>BBa_K399001 short</partinfo>
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===Source===
 
===Source===
  
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The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University. 
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mStrawberry was developed by Roger Tsien<sup>1</sup>
  
 
===References===
 
===References===
 +
1.  Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572

Revision as of 03:36, 28 October 2010

mStrawberry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.


Source

mStrawberry was developed by Roger Tsien1

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572