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| + | {{:Templates/Cambridge-fireflycolour|colour=red|substituion=Gly326Ser}} |
| − | {{Template:K325219 page header}} | + | |
| − | {{Template:K325219 Characterization Navbar}}
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| − | <div style="padding: 00px; width: 680px; border: 0px solid #000000;">
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| − | '''Description'''<br>
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| − | This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219]. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase]. Then a Gly326Ser mutation was used to create a red bioluminescence.
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| − | THIS PAGE IS CURRENTLY BEING UPDATED.
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| − | ==Pictures==
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| − | [[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']]
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| − | </center>
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| − | <sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
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| − | '''Compatibility'''<br>
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| − | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
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| − | [[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
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| − | </div>
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| − | '''References'''<br>
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| − | [http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1]:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''', 6-17.
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| − | </div>
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| − | [http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2]:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16), 372-376.
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| − | [http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3]:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39), 36508-36513.
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Revision as of 23:29, 26 October 2010
This part should show similarity to BBa_K325219 [[Part:BBa_K325{{{id}}}:Experience |Add Data]]
Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a red bioluminescence.
Colour of emission
Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
