Difference between revisions of "Part:BBa K365002"
| Line 10: | Line 10: | ||
<br><br> | <br><br> | ||
<center> | <center> | ||
| − | <img src="https://static.igem.org/mediawiki/2010/e/e6/ESBS-Strasbourg-PhyB900-black.png" width="250px" height="167px" border="0"> | + | <img src="https://static.igem.org/mediawiki/2010/e/e6/ESBS-Strasbourg-PhyB900-black.png" width="250px" height="167px" border="0"><br> |
<a target="_blank" href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/visual"><font>You can visit our wiki "visual description" page for more info</font></a> | <a target="_blank" href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/visual"><font>You can visit our wiki "visual description" page for more info</font></a> | ||
</center> | </center> | ||
Revision as of 22:03, 26 October 2010
Phytochrome B from A. thaliana (aa 1-908)
This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.
Background:
Phytochromes characterised by a red/far-red photochromicity. Through red-light (650–670 nm) absorption the phytochrome undergoes a rapid conformational change from its ground state Pr to its active state Pfr. The structural change allows the binding of the PIF. This light-sensitive interaction has been mapped to the 650-residue amino-terminal photosensory core of PhyB (Khanna et al., 2004). The process is completely reversible through absorption in the near infra-red spectrum (705–740 nm).The photoreceptor protein PhyB serves for the light-dependent activation of the system, therefore it will be fused to the N-teminal of the ClpX-trimer.
You can visit our wiki "visual description" page for more info
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 490
Illegal BamHI site found at 572
Illegal XhoI site found at 523
Illegal XhoI site found at 542
Illegal XhoI site found at 2677 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2062
Illegal BsaI site found at 2468
Illegal SapI site found at 739
Conception:
In in-vivo applications it has been shown that the PIF-interaction with the PhyB photosensory core (residues 1–650) is irreversible in infrared light. Lim & Voigt (2009) demonstrated by assaying PIF6 (which has the strongest interactions of all previously reported PIF domains) against different variants of PhyB that the tandem C-terminal PAS domains (residues 1-908)of plant phytochromes are necessary to confer rapid photoreversibility under infrared light. The original sequence contains a SpeI restriction within the first 908 residues.