Difference between revisions of "Part:BBa K404250"

Revision as of 21:48, 26 October 2010

pCMV_[AAV2]-VP123ex (ViralBrick-587-Beta-lactamase)
ß-Lactamase motif, inserted into the 453 loop of pCMV_[AAV2]-VP123

Usage & biology

ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides' N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place. Assembled capsids can easily be detected by a beta-lactamase assay. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2455
Illegal BamHI site found at 2396
Illegal XhoI site found at 698
Illegal XhoI site found at 884
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 665
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3075
Illegal BsaI site found at 3711
Illegal SapI site found at 1833

@@ Line 6: / Line 6: @@
 [[Image:Freiburg10_pCMV_VP123 587-BLA.png|thumb|center|480px]]<br>
-<!-- Add more about the biology of this part here
+===Usage & biology===
-===Usage and Biology===
+ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides' N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place.
+Assembled capsids can easily be detected by a beta-lactamase assay.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>