Difference between revisions of "Part:BBa K404235"
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Revision as of 19:50, 26 October 2010
[AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)
The Z34C antibody binding motif, inserted into the 453 loop of [AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAless
Usage and Biology
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3713
Illegal XhoI site found at 1913
Illegal XhoI site found at 2099 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4239
Illegal BsaI site found at 4421
Illegal BsaI site found at 4458
Illegal SapI site found at 3048