Difference between revisions of "Part:BBa K364319"

Latest revision as of 19:50, 26 October 2010

Gal4 DBD

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.

More on GAL4 DNA binding domain: [http://en.wikipedia.org/wiki/Two-hybrid_screening].

Uniprot GAL4 DNA binding domain: [http://www.uniprot.org/uniprot/P04386].

View GAL4 DNA binding domain crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=3COQ&bionumber=1].

3D structure of dimerized Gal4 DNA binding domain.

Description of the RXRa DBD crystal structure with reference here:[http://www.cell.com/structure/retrieve/pii/S0969212608001809].

Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.

Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 218
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 137

@@ Line 11: / Line 11: @@
 View GAL4 DNA binding domain crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=3COQ&bionumber=1].
-  [[Image:GAL4.jpg|800px|thumb|center|3D structure of dimerized Gal4 DNA binding domain]]
+  [[Image:GAL4.jpg|800px|thumb|center|3D structure of dimerized Gal4 DNA binding domain.]]
 Description of the RXRa DBD crystal structure with reference here:[http://www.cell.com/structure/retrieve/pii/S0969212608001809].