Difference between revisions of "Part:BBa K404208"

Revision as of 18:11, 26 October 2010

ViralBrick-587-Beta Lactamase (BLA)

Usage and Biology

ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place. Assembled capsids can easily be detected by a beta-lactamase assay.

Restriction sites

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 587	[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]		100;50;10;100	37°C
downstream 587	[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]		0;25;0;100	37°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 10
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place.
-Assembled capsids can easily be detected by beta-lactamase assay.
+Assembled capsids can easily be detected by a beta-lactamase assay.
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